Dr. Daniel Forciniti

   Missouri University of Science and Technology
   210-I Bertelsmeyer Hall
   Rolla, MO 65409
   Phone: 573-341-4427
   E-mail: forcinit@mst.edu

   BS Chem, University of Buenos Aires, Argentina, 1979
   MS ChE, University of the South, Argentina, 1984
   PhD ChE, North Carolina State University, 1991

Research Areas

Molecular simulations of biological molecules at liquid/solid interfaces, light and neutron scattering of biological molecules, the molecular basis of disease, purification of biomacromolecules

Research Statement

Peptide and proteins at interfaces: The main focus of our current research efforts in this area is the study of peptides at solid/liquid interfaces.  Both experimental and theoretical approaches are pursed in our group.  The use of dynamic and static light scattering for the study of proteins and tailored peptides in solution is routine in our laboratory.  This work is supplemented by neutron diffraction and neutron reflectivity studies performed at Oak Ridge National Laboratory.  The experimental work is supported (and interpreted) by using atomic level molecular simulations.  We are currently focusing our efforts on the study of surface-induced polypeptide aggregation.  This work is aimed at the understanding of the formation of amyloid deposits.

Purification of biomacromolecules:  The focus of our current research in this area is the study of the effect of glycosylation on the stability of monoclonal antibodies and other proteins.  A key component of our work is the experimental determination of second virial coefficients and their interpretations using statistical thermodynamics to quantify the effect of glycan groups on the potential of mean force of biomacromolecules.

Awards and Honors

The Whitaker Foundation Biomedical Engineering Award, 1995

Representative Publications

D. Forciniti.  Industrial Bioseparations:  Principles and Practice.  Blackwell Publishing/Wiley, Ames, Iowa, 309 pp

J.-W. Lee and D. Forciniti, "Purification of Human Antibodies from Transgenic Corn Using Aqueous Two-Phase Systems",  Biotechnology Progress 26, 159–167 (2010).

D. Forciniti, "Teaching a Bioseparations Laboratory:  From Training to Applied Research",  Journal of Chemical Engineering Education 43(4), 279–286 (2009)

A. Mungikar and D. Forciniti, "Effect of Co-solvents on the Adsorption of Peptides at the Solid-Liquid Interface", Biomacromolecules 7(1), 239–251 (2006).

W. H. Hamilton and D. Forciniti, "Surface Enrichment of Proteins at Quartz/Water Interfaces: A Neutron Reflectivity Study", Journal of Colloid and Interface Science 285(2), 458–468 (2005).

A. Mungikar and D. Forciniti, "Conformational Changes of Peptides at Solid/Liquid Interfaces:  A Monte Carlo Study", Biomacromolecules 5(6), 2147-2159 (2004).